PURExpressRF123 Kit from New England Biolabs

Product Specs
ItemPURExpressRF123 Kit
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberE6850S
Quantity10 reactions
ApplicationsProteomics & Glycomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-80 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Initiation at AUGUG and GUGUG sequences can lead to translation of overlapping reading frames in E. coliNucleic Acids ResearchJanuary 11, 2023Maximilian P Kohl, Maria Kompatscher, Nina Clementi, Lena Holl, Matthias D Erlacherdependence of translation was determined employing the PURExpress ΔRF123 in vitro translation system (NEB, E6850S) according to the provided protocol. As template, 30 ng of the corresponding plasmids were added to

(2) Chaperone requirements for de novo folding of Saccharomyces cerevisiae septinsMolecular biology of the cellOctober 1, 2022Hassell D, Denney A, Singer E, Benson A, Roth A, Ceglowski J, Steingesser M, McMurray M.reader (Cytation 3; BioTek). In vitro translation The PURExpress ΔRF123 kit (New England Biolabs #E6850S) was used with the following modifications from the manufacturer’s instructions. As described previously

(3) WNK1 is an assembly factor for the human ER membrane protein complexMolecular cellJuly 1, 2021Pleiner T, Hazu M, Tomaleri GP, Januszyk K, Oania RS, Sweredoski MJ, Moradian A, Guna A, Voorhees RM.2010",N/A Canine rough microsomes,"Walter and Blobel, 1983",N/A PURExpress ΔRF123 Kit,New England Biolabs,Cat# E6850S TransIT-293 transfection reagent,Mirus,Cat# MIR2705 Lipofectamine 3000,Thermo Scientific,Cat# L3000015 RNAiMAX

(4) A synthetic tRNA for EF-Tu mediated selenocysteine incorporationin vivoandin vitroFEBS LettersAugust 4, 2015Corwin Miller, Markus J. Bröcker, Laure Prat, Kevan Ip, Napon Chirathivat, Alexander Feiock, Miklós Veszprémi, Dieter Söllwere conducted using the PURExpress in vitro Protein Synthesis Kit (E6800S) or PURExpress ΔRF123 kit (E6850S, New England Biolabs Inc.), as noted. Reactions were prepared according to the manufacturer’s instructions

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